DMEM High Glucose Medium (without Phenol Red), IC-2205-S

Product Introduction

Dulbecco''''s Modified Eagle Medium (DMEM) was developed based on MEM (Minimum Essential Medium). Compared to MEM, DMEM contains a 2-fold increase in amino acids and a 4-fold increase in vitamins, along with the addition of non-essential amino acids, trace iron ions, and sodium pyruvate. DMEM was initially designed with a glucose concentration of 1000 mg/L (low-glucose type), and a formulation with 4500 mg/L glucose (high-glucose type) was later developed. It is now widely used for the culture of various cell types.

DMEM high-glucose medium is commonly used for fast-growing and loosely adherent cells, hybridoma myeloma cells, cloned cells, DNA-transfected transformed cells, various primary virus host cells, single cell culture, and vaccine production, such as EPO expression using CHO cells and hepatitis B vaccine production.

Phenol red is used as a pH indicator in culture medium to continuously monitor the pH. At low pH, phenol red turns the medium yellow; at high pH, it turns purple; at pH 7.2-7.4, it appears red, which is optimal for cell culture. However, phenol red has certain limitations. Studies have shown that phenol red can mimic the effects of steroid hormones (particularly estrogen); therefore, phenol red-free medium is recommended when working with estrogen-sensitive cells such as mammary tissue. Phenol red can also interfere with flow cytometry detection. Additionally, the presence of phenol red in some serum-free medium formulations may interfere with sodium-potassium balance.

This product contains amino acids, vitamins, inorganic salts, and other components necessary for various cell culture types, but does not contain proteins, lipids, or growth factors. Therefore, this product must be supplemented with serum or serum-free additives before use.

Product Features

1. High Glucose Formulation: Contains 4500 mg/L D-glucose, suitable for high-density cultures and rapidly proliferating cells.

2. Phenol Red-Free: Eliminates potential estrogenic effects and interference with flow cytometry analysis.

3. Complete Nutrient Profile: Includes 4 mM L-glutamine, 1 mM sodium pyruvate, and 3700 mg/L NaHCO₃ for comprehensive cell support.

4. Stringent Quality Control: Tested negative for bacteria, fungi, and mycoplasma contamination; endotoxin levels <3 EU/mL.

5. Sterile-Filtered: 0.1 µm filtered to ensure sterility for sensitive cell culture applications.

Specifications

Size: 500 mL

Product Form: Liquid

Concentration: 1¡Á

pH: 7.0-7.6

D-Glucose: 4500 mg/L

L-Glutamine: 4 mM

Sodium Pyruvate: 1 mM

NaHCO₃: 3700 mg/L

Phenol Red: -

HEPES: -

Endotoxin: <3 EU/mL

Sterility: Negative for bacteria, fungi, and mycoplasma

Storage and Stability

Storage Conditions: Store at 2-8¡ãC, protected from light. Do not freeze.

Shipping Conditions: Ambient temperature

Shelf Life: The product is stable for 18 months from the date of manufacture when stored as directed. After opening, use within 4-6 weeks when stored properly and handled aseptically.

Protocol (For Reference Only)

Important: Use aseptic technique when handling medium to prevent contamination. Warm medium to 37¡ãC before use. This product has been sterilized by 0.1 µm filtration; maintain sterile technique during use.

1. Preparation of Complete Medium: Supplement DMEM High Glucose Medium (without Phenol Red) with fetal bovine serum to a final concentration of 5-10% and add antibiotics (e.g., penicillin-streptomycin) as required for specific cell lines. Mix gently by swirling.

2. Medium Pre-warming: Place the required volume of complete medium in a 37¡ãC water bath or incubator for 15-30 minutes before use.

3. Cell Feeding: Aspirate spent medium from the culture vessel, replace with fresh pre-warmed complete medium, and return cells to the incubator maintained at 37¡ãC with 5% CO₂.

4. Subculturing: When cells reach 80-90% confluency, detach adherent cells using trypsin-EDTA, neutralize with complete medium, centrifuge if required, and resuspend in fresh complete medium for seeding into new vessels.

5. Monitoring: Without phenol red, pH changes are not visually indicated. Monitor cell morphology and growth regularly and replace medium every 2-3 days.

Precautions

1. This product is for research use only and is not intended for diagnostic or therapeutic use.

2. This product has been sterilized by 0.1 µm filtration; handle aseptically to avoid contamination.

3. To maintain optimal performance, do not freeze-thaw this product. Store at 2-8¡ãC protected from light.

4. This formulation does not contain phenol red; pH changes are not visually indicated. Establish a regular medium change schedule.

5. Supplemented complete medium should be used within 2-4 weeks when stored at 4¡ãC as glutamine degrades over time.

FAQ (Simplified)

Q1: Why choose phenol red-free DMEM?

A1: Phenol red-free medium is recommended for estrogen-sensitive cell types, flow cytometry applications, and studies where phenol red may interfere with detection or cellular responses.

Q2: What is the difference between DMEM high-glucose and low-glucose?

A2: DMEM high-glucose contains 4500 mg/L D-glucose, suitable for rapidly dividing cells and high-density cultures. Low-glucose DMEM contains 1000 mg/L D-glucose, preferred for primary cells and metabolically sensitive cultures.

Q3: Does this medium contain L-glutamine?

A3: Yes. This formulation contains 4 mM L-glutamine. For long-term culture, additional supplementation may be beneficial as glutamine degrades over time.

Q4: How should I monitor pH without phenol red?

A4: Without phenol red, pH cannot be visually monitored. Rely on regular medium changes and monitor cell growth and morphology as indicators of culture health.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of culture conditions including serum concentration and supplementation is recommended for specific cell lines.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data resulting from product use or storage.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-2205-S

Product Name: DMEM High Glucose Medium (without Phenol Red)

Size: 500 mL

Suggested Retail Price: CNY ¥320.00 / USD $32.00 / EUR €38.40 / JPY ¥5760.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.