McCoy''''s 5A Medium, IC-2035-S
Product Introduction
This product is a sterile, ready-to-use McCoy''''s 5A medium, a widely used basal cell culture medium originally developed by McCoy and colleagues for the in vitro cultivation of Novikoff hepatoma cells. McCoy''''s 5A has since become a standard medium for the culture of a variety of primary cells, established cell lines, and explants derived from human and animal tissues. The medium is characterized by its relatively high glucose concentration (3000 mg/L), the inclusion of a comprehensive panel of amino acids and vitamins, and supplementation with reduced glutathione and bacto-peptone. McCoy''''s 5A is particularly well-suited for supporting the growth of fastidious cell types, including primary fibroblasts, chondrocytes, osteoblasts, and endothelial cells. It is also commonly used for clinical cytogenetics applications, including the culture of amniotic fluid cells, chorionic villus samples, and bone marrow specimens for karyotyping and chromosomal analysis. The medium contains 1.5 mM L-glutamine, 2200 mg/L sodium bicarbonate, 3000 mg/L D-glucose, and 10 mg/L phenol red as a pH indicator, and is formulated without HEPES. This product is manufactured under stringent quality control conditions with endotoxin levels <1 EU/mL and is tested negative for bacteria, fungi, and mycoplasma.
Product Features
1. Optimized for Fastidious Cell Types: Specifically formulated to support the growth of primary cells and established cell lines with complex nutritional requirements.
2. High Glucose Concentration: Contains 3000 mg/L D-glucose, providing abundant energy substrate for high-density cultures and metabolically active cells.
3. Clinical Cytogenetics Compatible: Widely used for amniotic fluid cell, chorionic villus, and bone marrow culture for karyotyping applications.
4. Stringent Quality Control: Tested negative for bacteria, fungi, and mycoplasma contamination; endotoxin levels certified <1 EU/mL.
5. Sterile-Filtered: 0.1 µm filtered to ensure sterility for sensitive cell culture applications.
Specifications
Size: 500 mL
Product Form: Liquid
Concentration: 1¡Á
pH: 7.2-7.4
D-Glucose: 3000 mg/L
L-Glutamine: 1.5 mM
NaHCO₃: 2200 mg/L
Phenol Red: 10 mg/L
HEPES: -
Endotoxin: <1 EU/mL
Sterility: Negative for bacteria, fungi, and mycoplasma
Storage and Stability
Storage Conditions: Store at 2-8¡ãC, protected from light. Do not freeze.
Shipping Conditions: Ambient temperature
Shelf Life: The product is stable for 18 months from the date of manufacture when stored as directed. After opening, use within 4-6 weeks when stored properly and handled aseptically.
Protocol (For Reference Only)
Important: Use aseptic technique when handling medium to prevent contamination. Warm medium to 37¡ãC before use for optimal cell growth. This product has been sterilized by 0.1 µm filtration; maintain sterile technique during use.
1. Preparation of Complete Medium: Supplement McCoy''''s 5A Medium with fetal bovine serum to a final concentration of 5-20% and add antibiotics (e.g., penicillin-streptomycin) as required for specific cell types. Mix gently by swirling.
2. Medium Pre-warming: Place the required volume of complete medium in a 37¡ãC water bath or incubator for 15-30 minutes before use to minimize thermal shock to cells.
3. Cell Feeding: Aspirate spent medium from the culture vessel, replace with fresh pre-warmed complete McCoy''''s 5A Medium, and return cells to the incubator maintained at 37¡ãC with 5% CO₂.
4. Subculturing: When cells reach 80-90% confluency, detach adherent cells using trypsin-EDTA, neutralize with complete medium, centrifuge if required, and resuspend in fresh complete McCoy''''s 5A Medium for seeding into new vessels.
5. Cytogenetics Applications: For amniotic fluid cell culture, centrifuge amniotic fluid at 200 x g for 10 minutes, resuspend the cell pellet in complete McCoy''''s 5A Medium supplemented with 20-30% fetal bovine serum, and seed into appropriate culture vessels. Monitor for colony formation and process for chromosome analysis according to standard cytogenetics protocols.
6. Monitoring: Observe phenol red color indicator daily to assess pH changes; medium should appear pink-red at physiological pH and turn yellow-orange as acidity increases from cellular metabolism.
Precautions
1. This product is for research use only and is not intended for diagnostic or therapeutic use.
2. This product has been sterilized by 0.1 µm filtration; handle aseptically to avoid contamination.
3. To maintain optimal performance, do not freeze-thaw this product. Store at 2-8¡ãC protected from light.
4. This formulation does not contain HEPES; ensure adequate CO₂ atmosphere (5%) for proper pH maintenance during culture.
5. Supplemented complete medium should be used within 2-4 weeks when stored at 4¡ãC as glutamine degrades over time.
FAQ (Simplified)
Q1: What cell types are typically cultured in McCoy''''s 5A Medium?
A1: McCoy''''s 5A is widely used for primary fibroblasts, chondrocytes, osteoblasts, endothelial cells, and various established cell lines. It is also extensively used in clinical cytogenetics for amniotic fluid cell culture, chorionic villus sampling culture, and bone marrow culture for karyotyping applications.
Q2: Does this medium contain L-glutamine?
A2: Yes. This formulation contains 1.5 mM L-glutamine. For long-term culture or glutamine-sensitive cell types, additional supplementation with L-glutamine or stable glutamine substitutes may be beneficial.
Q3: What is the recommended serum concentration for cell culture with McCoy''''s 5A?
A3: The recommended serum concentration varies by cell type. Most established cell lines grow well in McCoy''''s 5A supplemented with 5-10% fetal bovine serum. Primary cells and cytogenetics applications may require 15-30% fetal bovine serum. Optimize serum concentration for specific applications.
Q4: Can this medium be used for suspension cell culture?
A4: While McCoy''''s 5A is primarily designed for adherent cell culture, it may be used for suspension-adapted cell lines with appropriate supplementation. Verify compatibility with your specific suspension cell line by pilot experiments.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of culture conditions including serum concentration and supplementation is recommended for specific cell lines.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data resulting from product use or storage.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-2035-S
Product Name: McCoy''''s 5A Medium
Size: 500 mL
Price: CNY ¥330.00 / USD $33.00 / EUR €39.60 / JPY ¥5940.00
2023 Version
