Nuclease-Free Water (DEPC-Treated), IC-5301
Product Introduction
This product is a high-purity, nuclease-free water treated with diethyl pyrocarbonate (DEPC) and subsequently autoclaved to remove residual DEPC. It is specifically designed for use in RNA and DNA applications where the presence of RNases and DNases must be rigorously excluded to prevent nucleic acid degradation. DEPC treatment inactivates RNase enzymes by covalently modifying histidine residues, providing robust protection against RNase contamination. Following DEPC treatment, the water is autoclaved to hydrolyze residual DEPC into ethanol and CO₂, ensuring the water is non-toxic and compatible with downstream enzymatic reactions including reverse transcription, PCR, in vitro transcription, and nucleic acid storage. This product is manufactured under stringent quality control conditions and is tested for the absence of non-specific endonuclease, exonuclease, and RNase activity.
Product Features
1. DEPC-Treated and Autoclaved: Provides rigorous inactivation of RNase and DNase enzymes while ensuring removal of residual DEPC for compatibility with enzymatic reactions.
2. Nuclease-Free Certified: Tested negative for non-specific endonuclease, exonuclease, and RNase activity.
3. Ready-to-Use: Supplied sterile and ready for immediate use without additional treatment or preparation.
4. Versatile Molecular Biology Grade: Suitable for RNA work, cDNA synthesis, qPCR, in vitro transcription, and all standard molecular biology applications.
5. High Purity: Ultrapure water processed through deionization, DEPC treatment, and autoclaving to ensure consistently high quality.
Specifications
Size: Available in 100 mL and 500 mL
Quality Control: Tested for absence of non-specific endonuclease, exonuclease, and RNase activity
Resistivity: ¡Ý18 M¦¸¡¤cm at 25¡ãC
Conductivity: ¡Ü0.055 µS/cm
Storage and Stability
Storage Conditions: Store at room temperature (15-25¡ãC). Protect from contamination by using aseptic technique when handling.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed in unopened packaging. After opening, use within 6 months when handled aseptically.
Protocol (For Reference Only)
Important: Use aseptic technique and wear gloves when handling to prevent nuclease contamination from skin and laboratory surfaces. Aliquot into smaller volumes for frequent use to minimize contamination risk.
1. RNA Reconstitution and Dilution: Use Nuclease-Free Water to reconstitute RNA pellets following precipitation or column purification, and to dilute RNA samples to desired concentrations for downstream applications.
2. cDNA Synthesis: Use Nuclease-Free Water as the diluent for preparing RNA templates, primer mixes, and master mixes in reverse transcription reactions.
3. PCR and qPCR Setup: Use Nuclease-Free Water as the aqueous component for preparing PCR and qPCR reaction master mixes, adjusting reaction volumes, and diluting primers and probes.
4. In Vitro Transcription: Use Nuclease-Free Water for preparing transcription reaction mixes, diluting RNA polymerases, and resuspending in vitro transcribed RNA products.
5. Enzymatic Reaction Preparation: Use Nuclease-Free Water as the universal diluent for all nuclease-sensitive enzymatic reactions including restriction digests, ligations, and nucleic acid labeling procedures.
Precautions
1. Use only certified nuclease-free pipette tips and tubes when handling this product; standard laboratory plasticware may introduce RNase contamination.
2. Aliquot the required volume into a separate sterile tube rather than directly pipetting from the stock bottle to minimize contamination risk.
3. Wear clean gloves and change them frequently when working with RNA samples and this product.
4. This product is DEPC-treated and autoclaved; it is non-toxic and compatible with all standard enzymatic reactions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: Is this product compatible with enzymatic reactions?
A1: Yes. After DEPC treatment, the water is autoclaved to hydrolyze residual DEPC into ethanol and CO₂, rendering it completely compatible with reverse transcription, PCR, in vitro transcription, and all other standard enzymatic reactions.
Q2: How should I store and handle this product to maintain nuclease-free status?
A2: Store at room temperature and handle using aseptic technique. Always use certified nuclease-free pipette tips and tubes. Aliquot working volumes into separate sterile tubes to avoid repeated pipetting from the stock bottle.
Q3: What is the difference between DEPC-treated water and non-DEPC treated nuclease-free water?
A3: DEPC treatment provides an additional level of RNase inactivation through covalent modification of histidine residues. Non-DEPC treated nuclease-free water relies on ultrafiltration or other methods. For highly sensitive RNA applications, DEPC-treated water is the gold standard.
Q4: Can I use this product for cell culture?
A4: No. This product is intended for molecular biology applications only and is not a tissue culture grade reagent. For cell culture applications, use a dedicated tissue culture grade water or sterile molecular biology grade water specifically validated for cell culture use.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, maintenance of nuclease-free technique is the responsibility of the user.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-5301
Product Name: Nuclease-Free Water (DEPC-Treated)
Size: 100 mL / 500 mL
Price:
100 mL: CNY ¥200.00 / USD $20.00 / EUR €24.00 / JPY ¥3600.00
500 mL: CNY ¥600.00 / USD $60.00 / EUR €72.00 / JPY ¥10800.00
2023 Version
