Tris-Gly SDS Running Buffer, 10X, IC-7907
Product Introduction
This product is a 10X concentrated stock solution of Tris-Glycine-SDS (TGS) running buffer, the standard electrophoresis buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). When diluted to 1X working concentration, the buffer provides the ionic strength, pH buffering capacity, and SDS detergent concentration necessary for the denaturing electrophoretic separation of proteins by molecular weight. Tris-Glycine-SDS buffer is the most widely used running buffer system for Laemmli SDS-PAGE, compatible with a broad range of polyacrylamide gel percentages and suitable for routine protein analysis, Western blotting sample preparation, and Coomassie staining workflows. The 10X concentrated formulation provides convenience for long-term storage and allows rapid preparation of working solutions by simple dilution with deionized water, reducing preparation time and ensuring lot-to-lot consistency.
Product Features
1. Standard Laemmli Buffer System: Optimized 10X formulation of the classic Tris-Glycine-SDS running buffer for denaturing SDS-PAGE.
2. Ready-to-Dilute Concentrate: Supplied as a sterile-filtered 10X stock requiring only 1:10 dilution with deionized water for 1X working solution preparation.
3. High Reproducibility: Consistent buffer composition eliminates variability associated with manual powder weighing and reconstitution.
4. Broad Compatibility: Suitable for use with Tris-Glycine gels of all standard polyacrylamide percentages and compatible with downstream Western blotting, staining, and gel drying procedures.
5. Convenient Storage: 10X concentrated format minimizes storage footprint and extends shelf life.
Specifications
Size: 500 mL
Formulation: 10X Concentrate (250 mM Tris, 1.92 M Glycine, 1% SDS, pH 8.3-8.5)
Storage and Stability
Storage Conditions: Store at room temperature (15-25¡ãC). Protect from light.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. If SDS precipitates during cold storage, warm the bottle to room temperature and mix gently before use.
Protocol (For Reference Only)
Important: Ensure complete dissolution of any precipitated SDS prior to dilution. Prepare 1X working solution fresh for optimal results.
1. Preparation of 1X Working Solution: Dilute the 10X Tris-Gly SDS Running Buffer 1:10 with deionized water (e.g., 100 mL of 10X buffer plus 900 mL of deionized water) and mix thoroughly by stirring or inversion to obtain 1X running buffer.
2. Electrophoresis Assembly: Assemble the gel cassette(s) in the electrophoresis tank according to the manufacturer''''s instructions, load protein samples and molecular weight markers into the gel wells, and fill the inner buffer chamber completely with 1X running buffer.
3. Buffer Addition: Pour 1X running buffer into the outer buffer chamber to the recommended fill level indicated on the electrophoresis tank.
4. Electrophoresis: Connect the tank to the power supply and run the gel using appropriate voltage and time settings for the specific gel percentage and protein size range (e.g., 120-180 V constant voltage for 45-90 minutes for a standard 10-12% gel).
5. Post-Electrophoresis: After the dye front reaches the bottom of the gel, discontinue electrophoresis, disassemble the gel cassette, and proceed to gel staining, Western transfer, or desired downstream application.
Precautions
1. If SDS has precipitated during storage at low temperatures, allow the bottle to equilibrate to room temperature and mix gently by inversion until the precipitate fully dissolves before dilution.
2. Do not use the 10X concentrate directly for electrophoresis; always dilute to 1X working concentration before use.
3. Use freshly prepared 1X running buffer for optimal and reproducible separation; 1X buffer may be reused 1-2 times but buffer depletion may affect migration patterns.
4. Avoid prolonged exposure of the buffer to direct sunlight to prevent photo-degradation of SDS.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What should I do if I observe white precipitate in the 10X concentrate?
A1: SDS precipitation commonly occurs when the buffer is stored at low temperatures. Warm the bottle to room temperature (20-25¡ãC) and mix gently by inversion or stirring until the precipitate completely dissolves. The buffer is fully functional after dissolution.
Q2: Can I reuse the 1X running buffer?
A2: 1X running buffer may be reused 1-2 times for routine SDS-PAGE. However, buffer ion depletion and pH changes may affect protein migration. For high-sensitivity applications such as quantitative Western blotting, use freshly prepared buffer.
Q3: What is the recommended running voltage for SDS-PAGE with this buffer?
A3: Typical running conditions are 120-180 V constant voltage for 45-90 minutes for a standard 10-12% Tris-Glycine gel. Adjust voltage and run time according to gel percentage and desired protein resolution.
Q4: Is this buffer compatible with gradient gels?
A4: Yes. Tris-Gly SDS Running Buffer is the standard buffer for all Tris-Glycine-based polyacrylamide gels, including linear gradient gels (e.g., 4-20%, 8-16%).
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of electrophoresis conditions is recommended for specific experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-7907
Product Name: Tris-Gly SDS Running Buffer, 10X
Size: 500 mL
Price: CNY ¥200.00 / USD $20.00 / EUR €24.00 / JPY ¥2250.00
2023 Version
