Recombinant Human EGFR (Epidermal Growth Factor Receptor)

Cat.No.:

CYT-P00533

Product Background

Recombinant Human EGFR

The EGFR subfamily of receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43% - 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.

Product Information

Source

Human Cells

Molecular Weight

69.6 KDa

AA Sequence

Recombinant Human Epidermal Growth Factor Receptor is produced by our Mammalian expression system and the target gene encoding Leu25-Ser645 is expressed with a 6His tag at the C-terminus.

Biological Activity

Loaded Anti-Human EGFR mAb-Fc on Pro A Biosensor, can bind Recombinant Human EGFR-His with an affinity constant of 7.23 nM as determined in BLI assay.

Physical Appearance

Lyophilized from a 0.2 ¦Ìm filtered solution of 20mM Tris-HCl, 8% Sucrose, 50mM Nacl, 0.05% Tween80, pH8.0.

Formulation

Lyophilized from a 0.2 ¦Ìm filtered solution of 20mM Tris-HCl, 8% Sucrose, 50mM
Nacl, 0.05% Tween80, pH8.0

Endotoxin

< 0.01 EU/µg as determined by LAL test.

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100¦Ìg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 ¡ãC as supplied.

1 month, 2 to 8 ¡ãC under sterile conditions after reconstitution.

3 months, -20 to -70 ¡ãC under sterile conditions after reconstitution.

Usage Information

This  product  is  offered  by  BioCytoSci only  for Scientific Research & Laboratory  USE. NOT FOR OTHER USE.

Order Information

Cata.No.

Quantity

Price($)

Price($)

Price(£¤)

CYT-P00533

10ug

$156.00

€171.60

£¤1,560.00

CYT-P00533

50ug

$460.00

€506.00

£¤4,600.00

CYT-P00533

1mg

$4,200.00

€4,620.00

£¤42,000.00

Free Delivery on orders over $200.00.

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