Recombinant Human TNF¦Á

Cat.No.:CYT-P01375

Product Background

Recombinant Human TNF alpha

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Product Information

Source

E. coli

Molecular Weight

17.5 kDa

AA Sequence

A DNA sequence encoding the human TNF-¦Á soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.

Biological Activity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 mm filtered concentrated solution in PBS, pH 7.4.

Endotoxin

Less than 1 EU/mg of rHuEGF as determined by LAL method.

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100¦Ìg/ml.Dissolve the lyophilized protein for best performance, we strongly recommend you to follow the reconstitution protocol provided in the Datasheet.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 ¡ãC as supplied.

1 month, 2 to 8 ¡ãC under sterile conditions after reconstitution.

3 months, -20 to -70 ¡ãC under sterile conditions after reconstitution.

Usage Information

This  product  is  offered  by  InCell-Cytokine only  for Scientific Research & Laboratory  USE. NOT FOR OTHER USE.

Order Information

Quantity

Size1

Size2

Size3

Size4

10ug

$80.00

€88.00

£¤800.00

£¤15,920.00

100ug

$412.00

€453.20

£¤4,120.00

£¤81,988.00

1mg

$1,650.00

€1,815.00

£¤16,500.00

£¤328,350.00

Free Delivery on orders over $200.00.

We Devoted Ourselves To The Development Of Biomedical Research Reagent.