Anti-Myc Tag Magnetic Beads

Cat.No.IC-8117

Product Description

Anti-MYC Tag Beads(MYC Tag Immunoprecipitation Magnetic Beads) are designed to specifically bind to proteins tagged with the MYC epitope, making them suitable for the immunoprecipitation (IP) of proteins, protein complexes, protein-nucleic acid complexes, and other antigens. This product is applicable for antigen samples derived from cell lysates, cell culture supernatants, serum, ascites, and other sources.

Usage

1. Preparation of Cell Lysate

Select an appropriate lysis buffer to treat the cell sample, prepare the cell lysate following standard procedures, and keep it on ice for immediate use or store at -20¡ãC for long-term preservation.

2. Magnetic Beads Pretreatment

2.1  Vortex the immunoprecipitation magnetic beads for 1 min to resuspend them, and transfer 25~50 µL of the bead suspension into a 1.5 mL EP tube.

2.2 Add 500 ¦ÌL of Washing Buffer to the EP tube for washing, gently invert the EP tube several times to resuspend the beads. Place the EP tube in a magnetic separator, let it stand for 1 min for magnetic separation, remove the supernatant, and take out the EP tube. Repeat the washing steps twice.

3. Immunoprecipitation Steps

3.1 Add 500 ¦ÌL of the prepared cell lysate to the EP tube, place the EP tube on a rotary mixer, and mix at 37¡ãC for 30 min. If the binding affinity is weak, incubate at room temperature for 1 h or at 4¡ãC overnight.

3.2 After incubation, perform magnetic separation, discard or save the supernatant for further analysis.

3.3 Add 500 ¦ÌL of Washing Buffer to the EP tube for washing. Perform magnetic separation, remove the supernatant, take out the EP tube, and repeat the washing of the beads three times.

4. Antigen Elution

Several antigen elution protocols are provided, users can choose different elution methods based on the needs of subsequent detection.

4.1 Denaturing Elution: This method is suitable for SDS-PAGE detection. Add 100 ¦ÌL of SDS-PAGE Loading Buffer (self-prepared) to the EP tube, mix well, heat at 95¡ãC for 5 min. Then perform magnetic separation or centrifugation (room temperature, 13000 g, 10 min), collect the supernatant for SDS-PAGE detection.

4.2 Acidic Elution: Add 100 ¦ÌL of Acidity Elution Buffer to the EP tube, incubate on a rotary mixer at 37¡ãC for 5-10 min. Then perform magnetic separation or centrifugation, collect the supernatant. If neutralization of the acidic eluent is needed, add 50 ¦ÌL of Neutralization Buffer to 100 ¦ÌL of eluent to adjust the pH to neutral.

Precautions

1. Avoid freezing the magnetic beads. Beads should be stored in storage solution to prevent drying.

2. To minimize bead loss, each magnetic separation should last no less than 1 min.

3. Before taking beads from the storage tube, ensure thorough vortexing for uniform suspension. Avoid bubble formation during operation.

4. It is recommended to use high-quality pipette tips and reaction tubes to avoid losses due to bead and solution adherence.

5. Operators can use the supernatants collected during the antibody binding and antigen binding steps to detect the binding of antibodies and antigens to the beads based on actual needs.

6. In IP experiments, the affinity of different types of antibodies and antigens varies. If the buffer system provided with this kit does not yield ideal experimental results, operators may screen and prepare their own buffers.

7. This product is strictly for scientific research by professionals only, not for clinical diagnosis or treatment, not for use in food or drugs, and should not be stored in ordinary residences.

8. For your safety and health, please wear lab coats and disposable gloves during operation.

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