CellProTM Serum-free Medium(SF1801-02)
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Introduction
CellProTM Serum-free Medium (cat. no. SF1801-02) is a serum-free medium designed for the cultivation of a wide variety of mammalian cell types. The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of CellProTM Serum-free Medium is approximately 3 mg/ml. CellProTM Serum-free Medium does not contain L-glutamine.
CellProTM Serum-free Medium has been used to grow a variety of cells of primary origin and established cell lines. The medium may be used to support the fusion of cells during the formation of hybridomas.CellProTM Serum-free Medium has been used to grow cells of lymphoid origin including monocyte and macrophage cell lines. Epithelial and fibroblastic cells have been cultivated in CellProTM Serum-free Medium and endothelial cells have been cultivated under reduced serum concentration (5% fetal bovine serum using CellProTM Serum-free Medium as a base formulation).
CellProTM Serum-free Medium has also been used as a complete medium to support the generation of viral particles for use in vaccine production.
Instructions for Use
CellProTM Serum-free Medium is a complete all-purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines. The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution per 500 ml.
Turbidity may develop CellProTM Serum-free Medium . Experiments have determined that the turbidity will not alter the performance of the product.
CellProTM Serum-free Medium should be stored at 2¡ãC - 8¡ãC. For cells in serum-containing medium weaning is NOT required.
Product Use Statement
THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.
Ordering Information
Catalog Description number
SF1801-02 CellProTM Serum-free Medium
Size 500 ml without L-glutamine
Partial List of Cells Cultivated with CellProTM Serum-free Medium :
Cell line |
Source |
Cell type |
HEL, N-10 |
Human |
Fetal lung diploid fibroblast |
HeLa |
Human |
Uterine cancer |
HuL-1,2 |
Human |
Liver (normal) |
HuK-1 |
Human |
Kidney (normal) |
HuS-1AT |
Human |
Skin |
HEC |
Human |
Embryonic cancer |
HL-60 |
Human |
Acute promyelocytic leukemia |
Raji |
Human |
Burkitt¡¯s lymphoma |
EB-3 |
Human |
Burkitt¡¯s lymphoma |
K-562 |
Human |
Chronic myelocytic leukemia |
HNK |
Human |
Neonatal kidney (primary) |
HTC29 |
Human |
Colon Cancer |
TT |
Human |
Medulary thyroid tumor |
MB231 |
Human |
Breast carcinoma |
U138 |
Human |
Glioma |
FM3A |
Mouse |
Breast cancer |
NS-1 |
Mouse |
Myeloma |
L |
Mouse |
Subcutaneous |
P388D1 |
Mouse |
Macrophage-like |
P815 |
Mouse |
Mast cell tumor |
T3 |
Mouse |
Pituitary |
B82 |
Mouse |
L cells ¨Cconnective tissue |
RPL-1 |
Rat |
Peritoneum |
RSP-2 |
Rat |
Spleen |
RLG-1 |
Rat |
Lung |
Lym-1 |
Rat |
Lymph node |
RCR-1 |
Rat |
Brain |
235-1, MNQ |
Rat |
Pituitary |
GC, GH3 |
Rat |
Pituitary |
CA77 |
Rat |
Medulary thyroid tumor |
Rat-1 |
Rat |
Fibroblast |
JTC-12 |
Monkey |
Kidney |
COS1, COS7 |
African green monkey |
SV40 transformed kidney |