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Human Cytokine
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PNGase F£¬CYT-P21163
Click£º10577     Release date£º2013-2-13    Author£ºAdministrator    Source£ºOriginal

PNGase F

 

 

Cat.No.:

CYT-P21163

 

 

 

Background

PNGase F

Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10 ¦Ìg) is deglycosylated by 2.4 ng rFmPNGase F within 60 minutes, as measured under the described conditions.Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins.

Information

Source

Escherichia coli.

Molecular Weight

Approximately 35KD.

AA Sequence

APADNTVNIKTFDKVKNAFGDGLSQSAEGTFTFPADVTTVKTIKMFIKNECPNKTCDEWDRY ANVYVKNKTTGEWYEIGRFITPYWVGTEKLPRGLEIDVTDFKSLLSGNTELKIYTETWLAKG REYSVDFDIVYGTPDYKYSAVVPVIQYNKSSIDGVPYGKAHTLGLKKNIQLPTNTEKAYLRTT ISGWGHAKPYDAGSRGCAEWCFRTHTIAINNANTFQHQLGALGCSANPINNQSPGNWAPDR AGWCPGMAVPTRIDVLNNSLTGSTFSYEYKFQSWTNNGTNGDAFYAISSFVIAKSNTPISAPV VTN

Unit Definition Assay

10 ¦Ìg of RNase B are denatured with 1X Glycoprotein Denaturing Buffer (0.5% SDS, 40 mM DTT) at 100¡ãC for 10 minutes. After the addition of NP-40 and GlycoBuffer 2, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37¡ãC. Separation of reaction products are visualized by SDS-PAGE.

Physical Appearance

Sterile Filtered Clear Solution..

Formulation

Supplied as a 0.2 ¦Ìm filtered solution of PBS, pH 7.4, 10% Glycerol.

Endotoxin

Less than 0.1 EU/mg of rHuEGF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20¡æ. Further dilutions should be made in appropriate buffered solutions.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Stability & Storage

It is recommended to freeze aliquots at -80¡ãC for extended storage. Avoid repeated freeze-thaw cycles.

Stored at -80¡æ for 1 year.

2 weeks, 2 to 8¡æunder sterile conditions after reconstitution.

It is stable at -20¡æ for 3 months after opening.

Usage Information

 

 

 

 

 

 

This  product  is  offered  by  BioCytoSci only  for Scientific Research & Laboratory  USE. NOT FOR OTHER USE.

Order Information

 

 

 

 

 

Cata.No.

Quantity

Price($)

Price($)

Availability

CYT-P21163

5,000 units

$156.00

€171.60

In stock

CYT-P21163

15,000 units

$296.00

€325.60

In stock

CYT-P21163

75,000 units

$1,300.00

€1,430.00

In stock

Free Delivery on orders over $200.00.

We Devoted Ourselves To The Development Of Biomedical Research Reagent.


InCellGene


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