BackGround
Exosomes are 30¨C150 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids, urine and in media from cells in culture. Exosomes contain distinct subsets of microRNAs depending upon the cell type from which they are secreted. Standardgrowth medium for most cells in culture require fetal bovine serum (FBS) as agrowth supplement to DMEM. FBS is derived from bovine (cow) serum and contains a high abundance of cow exosome vesicles. These exosomes can interfere or cause significant background issues when studying the exosomes secreted from your cells of interest in standard culture conditions.
ICGE LLC. has developed an Exosome-Free FBS called EF-FBSthat has beed stripped of bovine exosomes. Exo-FBS supports equivalent growth ofmany types of cells in culture, is devoid of cow CD63 positive exosomes and doesnot have any measurable bovine microRNAs. Perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes in your experiments.
Product Description
This product is derived from Uruguayan Premium Fetal Bovine Serum (FBS) and undergoes ultracentrifugation to efficiently remove exosomes, achieving a removal rate of over 99%. It is suitable for cell culture, exosome research, stem cell culture, and other experiments requiring exosome-free conditions.
Product Name: Exosome-Depleted Premium Fetal Bovine Serum (Exosome-Depleted FBS)
Source: Uruguayan Premium Fetal Bovine Serum (Uruguayan Premium FBS)
Exosome Removal Method: Ultracentrifugation
Exosome Removal Efficiency: >99%
Product Advantages
-High Removal Efficiency: Ultracentrifugation ensures minimal exosome residue (<1%).
-Premium Source Material: Uruguayan Premium FBS provides rich nutrients, ideal for sensitive cell cultures.
-Low Endotoxin Level: Endotoxin content ¡Ü10 EU/mL, reducing cytotoxicity risks.
Technical Specifications
- Exosome Removal Method: Ultracentrifugation (100,000¡Ág, 4¡æ, 18 hours)
- Serum Source: Healthy Uruguayan fetal bovine, free from BVDV, mycoplasma, and other contaminants
- Storage Conditions: Store frozen at -20¡æ, avoid repeated freeze-thaw cycles
- Shelf Life: 5 years (when stored at -20¡æ)
Applications
- Exosome isolation studies (ensures no interference from serum-derived exosomes)
- Stem cell and primary cell culture
- Cell function research (e.g., migration, proliferation, signaling pathways)
- Exosome functional studies (e.g., miRNA sequencing, proteomics)
Usage Instructions
1. Thaw slowly at 4¡æ before use; avoid rapid thawing at high temperatures.
2. Gently mix after thawing to prevent bubble formation.
3. Recommended ratio: Mix with basal medium at 10% (or as required).
4. Aliquot unused portions and store frozen to avoid repeated freeze-thaw cycles.
Precautions
1.This product retains native serum proteins and growth factors only exosomes are removed.
2.For exosome research, include an exosome-depleted serum negative control.
3.Avoid repeated freeze-thaw cycles to maintain serum activity.
Quality Control
Each batch undergoes the following tests:
- Sterility testing (bacterial/fungal culture)
- Mycoplasma detection (PCR method)
- Exosome residue detection (NTA or WB)
- Endotoxin testing (LAL method)
Disclaimer
Due to variations in cell types, users are advised to pre-test serum compatibility. The company is not liable for cell culture issues caused by improper handling.
Order Information
|
Cat./REF. |
Size |
Price($£© |
Price(€) |
Price(£¤/CNY£© |
Price(£¤/JYP£© |
|
IC-1909 |
50ml |
$199.00 |
€ 238.80 |
£¤1,990.00 |
£¤39,601.00 |
|
IC-1909 |
500ml |
$1,590.00 |
€ 1,908.00 |
£¤15,900.00 |
£¤316,410.00 |
