Agarose, Low EEO, IC-6088
Product Introduction
Agarose is a neutral polysaccharide component extracted from agar or agar-containing seaweed. Structurally, it is a linear polymer composed of alternating β-D-galactopyranose (1-4) linked 3,6-anhydro-α-L-galactopyranose units. Agarose dissolves in water when heated above 90°C and forms a semi-solid gel upon cooling to 35-40°C, a property that constitutes the primary basis for its diverse applications. Agarose gel performance is typically expressed as gel strength, with higher gel strength indicating superior gel properties.
This product is a low electroendosmosis (Low EEO) agarose suitable for routine nucleic acid analysis by gel electrophoresis and blotting. It provides reliable separation of DNA and RNA fragments with minimal electroendosmotic effects, making it an ideal choice for everyday molecular biology applications.
Product Features
1. Low EEO: Minimal electroendosmotic effects for reliable nucleic acid separation.
2. High Gel Strength: >1200 g/cm² (1% gel), ensuring durable and easy-to-handle gels.
3. Wide Application: Suitable for routine nucleic acid electrophoresis and blotting.
4. High Purity: Free of detectable nucleases and proteases.
5. Long Shelf Life: Stable for 5 years under recommended storage conditions.
Specifications
Size: 100 g
CAS Number: 9012-36-6
Molecular Formula: C12H18O9
Molecular Weight: 306.26
Appearance: White or off-white powder
Gel Strength (1%): >1200 g/cm²
Gelling Temperature (1.5%): 35-37°C
Melting Temperature (1.5%): 87-89°C
Nuclease: Not detected
Protease: Not detected
EEO (Electroendosmosis): Low
Storage and Stability
Storage Conditions: Store at room temperature (15-25°C) in a dry environment. Keep container tightly sealed.
Shelf Life: 5 years from the date of manufacture.
Protocol (For Reference Only)
Important: Use clean glassware and deionized water for gel preparation. Ensure complete dissolution of agarose before pouring gels.
1. Gel Preparation: Add the appropriate amount of agarose powder to 1× electrophoresis buffer (e.g., TAE or TBE) to achieve the desired concentration (typically 0.5-2.0% w/v).
2. Dissolution: Heat the mixture in a microwave oven or on a hot plate with stirring until the agarose is completely dissolved and the solution becomes clear. Swirl gently to ensure uniform mixing.
3. Cooling: Allow the solution to cool to approximately 55-60°C before adding nucleic acid stains (e.g., ethidium bromide, SYBR Safe) if desired.
4. Gel Casting: Pour the solution into a gel tray with an appropriate comb inserted. Allow the gel to set at room temperature for 30-45 minutes.
5. Electrophoresis: Submerge the gel in 1× electrophoresis buffer, load samples, and run at appropriate voltage.
Precautions
1. Avoid inhalation of agarose powder; handle in a well-ventilated area.
2. Ensure complete dissolution before casting gels; undissolved particles will compromise gel uniformity and resolution.
3. Use clean, dedicated glassware to avoid nuclease contamination.
4. Store in a tightly sealed container in a dry environment to prevent moisture absorption.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What does Low EEO mean?
A1: EEO (Electroendosmosis) refers to the movement of liquid through the gel matrix in response to an electric field. Low EEO agarose minimizes this effect, resulting in sharper bands and better resolution.
Q2: What gel percentage should I use for DNA separation?
A2: The recommended gel percentage depends on the size of DNA fragments: 0.5-0.7% for large fragments (5-20 kb), 1.0% for medium fragments (0.5-10 kb), and 1.5-2.0% for small fragments (100-1000 bp).
Q3: Can this agarose be used for RNA gels?
A3: Yes. This agarose is suitable for routine RNA electrophoresis, including denaturing formaldehyde gels. Ensure RNase-free conditions are maintained.
Q4: How should I store this product?
A4: Store at room temperature in a dry environment with the container tightly sealed. The product is stable for 5 years.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of gel concentration and electrophoresis conditions is recommended for specific applications.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-6088
Product Name: Agarose, Low EEO
Size: 100 g
Suggested Retail Price: CNY ¥850.00 / USD $85.00 / EUR �102.00 / JPY ¥15300.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
