CellPro^TM  0.25% Trypsin-EDTA(1¡Á),no Penol Red £¨IC-1709£©

Product Discription

This product is a sterile, ready-to-use 1X solution of 0.25% porcine trypsin with EDTA (0.02%) formulated without phenol red. Trypsin is a serine protease derived from porcine pancreas that cleaves peptide bonds on the carboxyl side of lysine and arginine residues, effectively detaching adherent cells from culture vessel surfaces. EDTA is a chelating agent that binds calcium and magnesium ions, disrupting cadherin-mediated cell-cell adhesion and integrin-mediated cell-matrix attachment, thereby enhancing trypsin-mediated cell dissociation. This trypsin-EDTA solution is widely used in routine cell culture for the subculturing of adherent mammalian cells, including fibroblasts, epithelial cells, and many established cell lines. The absence of phenol red eliminates potential estrogenic activity and interference with fluorescence-based assays, making this formulation suitable for applications where phenol red must be excluded.

Product Features

1. Standardized 0.25% Trypsin Concentration: Provides reproducible cell detachment for routine subculturing of adherent mammalian cell lines.

2. EDTA Supplementation: Enhances cell dissociation by chelating divalent cations required for cell adhesion, promoting efficient and uniform cell detachment.

3. Phenol Red-Free Formulation: Eliminates potential interference with fluorescence microscopy and estrogen-sensitive assays.

4. Ready-to-Use 1X Solution: Supplied as a sterile-filtered solution requiring no dilution, reducing preparation time and contamination risk.

5. Stringent Quality Control: Each lot is tested for sterility, pH, osmolality, and cell detachment performance.

Specifications

Size: 100 mL / 500 mL

Trypsin Concentration: 0.25% (w/v)

EDTA Concentration: 0.02% (w/v)

Source: Porcine pancreas

Phenol Red: Without

Form: 1X sterile-filtered solution

pH: 7.2-7.5

Storage and Stability

Storage Conditions: Store at -20¡ãC for long-term storage. Working aliquots may be stored at 4¡ãC for up to 2 weeks. Avoid repeated freeze-thaw cycles.

Shelf Life: The product is stable for 24 months from the date of manufacture when stored at -20¡ãC as directed.

Protocol (For Reference Only)

Important: Warm trypsin-EDTA solution to room temperature or 37¡ãC before use. Minimize exposure of cells to trypsin to avoid over-digestion and reduced viability. Monitor cell detachment under a microscope.

1. Preparation: Warm trypsin-EDTA solution to 37¡ãC in a water bath. Pre-warm complete culture medium for trypsin neutralization.

2. Washing: Aspirate culture medium from the cell monolayer. Wash cells once with sterile PBS without Ca²⁺/Mg²⁺ to remove residual serum, which contains trypsin inhibitors.

3. Trypsinization: Add sufficient trypsin-EDTA solution to cover the cell monolayer (typically 1-2 mL per T25 flask or 2-3 mL per T75 flask). Gently rock the flask to ensure complete coverage. Incubate at 37¡ãC for 2-5 minutes, monitoring cell detachment under a microscope.

4. Neutralization: Once cells are detached (appear rounded and are freely floating), add an equal or greater volume of complete culture medium containing serum to neutralize trypsin activity. Gently pipette to dissociate cell clumps into a single-cell suspension.

5. Centrifugation and Seeding: Transfer the cell suspension to a centrifuge tube and centrifuge at 200-300 x g for 5 minutes. Aspirate the supernatant, resuspend the cell pellet in fresh complete culture medium, and seed into new culture vessels at the desired density.

Precautions

1. Avoid prolonged exposure of cells to trypsin-EDTA as over-digestion may reduce cell viability and damage cell surface proteins.

2. The solution does not contain phenol red; monitor cell detachment under a microscope as pH changes are not visually indicated.

3. Do not use trypsin-EDTA at temperatures above 37¡ãC or for extended incubation periods beyond those required for complete cell detachment.

4. Some cell types may be sensitive to trypsin; for these cells, consider using lower trypsin concentrations or alternative detachment methods.

5. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: How does this trypsin solution differ from standard formulations?

A1: This formulation contains 0.25% trypsin with 0.02% EDTA and does not contain phenol red. The absence of phenol red eliminates potential interference with fluorescence-based assays and estrogen-sensitive cell lines.

Q2: How should I store this product?

A2: Store at -20¡ãC for long-term storage. After thawing, the solution may be stored at 4¡ãC for up to 2 weeks. Aliquot into smaller volumes to avoid repeated freeze-thaw cycles.

Q3: How do I know when cells are sufficiently detached without phenol red?

A3: Monitor cell detachment under a microscope. When cells appear rounded and detached from the surface, they are ready for neutralization. Gently tapping the flask can facilitate detachment.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of trypsinization time and conditions is recommended for specific cell types.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-1709

Product Name: 0.25% Trypsin-EDTA (1X), without Phenol Red

Size: 100 mL / 500 mL

Suggested Retail Price:

100 mL: CNY ¥225.00 / USD $22.50 / EUR €27.00 / JPY ¥4050.00

500 mL: CNY ¥900.00 / USD $90.00 / EUR €108.00 / JPY ¥16200.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.

 Product  Safety

InCellGene Trypsin 1X solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, Mycoplasma, Bacterial, Fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Product Use Statement

THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.

Order Information